MMI Live Cell Microdissection – The new mmi Live CellChamber
Introduction: The isolation and enrichment of individual live cells for culture and differentiation experiments or for their proteomic and genomic analysis is of increasing interest in stem cell research, cancer research and tissue engineering. While mechanical separation techniques are cumbersome, time consuming and bear the risks of contamination or mechanical stress, faster Laser Microdissection based methods are currently under development. The mmi CellCut PLUS in combination with the newly developed mmi Live CellChamber enables contamination-free isolation of cells in living culture.
Components of the new mmi Live CellChamber
Introduction: The isolation and enrichment of individual live cells for culture and differentiation experiments or for their proteomic and genomic analysis is of increasing interest in stem cell research, cancer research and tissue engineering. While mechanical separation techniques are cumbersome, time consuming and bear the risks of contamination or mechanical stress, faster Laser Microdissection based methods are currently under development. The mmi CellCut PLUS in combination with the newly developed mmi Live CellChamber enables contamination-free isolation of cells in living culture.
Components of the new mmi Live CellChamber - A membrane ring, for the initial cultivation of cells
- A cell culture dish, to house the membrane ring with seeded cells
- The microdissection chamber, UV – permeable and filled with adhesive for Laser Microdissection of single cells or group of cells
Note: All components are sterilized and ready to use
The Workflow Adherent cells are grown in a special metal ring on a membrane inside a petri dish. Once a desired cell density is reached, the membrane ring is transferred to the adhesive area of the microdissection chamber. The cell or cells of interest are selected and cut using Laser Microdissection. In addition, unwanted cells can be destroyed or ablated by using individual laser shots. The cell chamber is then removed and only the cells of interest remain in the microdissection chamber.
Repeated selection of wanted cells: This process can be repeated using the same metal ring in a new microdissection chamber to select and separate more than one cell type. Once isolation is complete, sufficient medium is added to the microdissection chamber and the cells can be re-cultivated.
Your Advantage
The isolation of single cells is a unique and highly useful tool in wide range of research and clinical applications, including individual cell analysis, clonal expansion, transfection experiments and stem cell research. Enzyme treatments (i.e. trypsin), other potentially harmful selective reagents, as well as time consuming repetitive enrichments can be avoided. The benefit is an increase in reliability, effectiveness, and accuracy of your research.
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Order Nr.: 50301
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