In developmental biology, many processes are not yet fully understood as the regulatory network and interplay of development-related transcription factors is very complex.
The study published by Cui et al. incorporated time as an additional dimension to 3D Geo-Seq data to uncover transcription factor activity at key developmental timepoints in germ layer specification and lineage differentiation.
The analysis of the spatial transcriptome of development-related transcription factors generated a series of molecular maps that depicted the developmental trajectory of four major progenitor populations during gastrulation that was not previously identified by conventional fate mapping.
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