APPLICATIONS

Spatial Transcriptomics

Mapping Gene Expression at Single-Cell Resolution

CellCut Laser Microdissection
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What is Spatial Transcriptomics? 

Spatial transcriptomics is a cutting-edge technique that maps gene expression within tissues while preserving spatial context. Unlike traditional RNA sequencing, it allows researchers to see exactly where each gene is active, down to single cells. This spatial resolution is essential for understanding tissue architecture, cellular interactions, and disease mechanisms.  

By combining single-cell analysis with spatial information, scientists can uncover subtle differences between neighbouring cells that would otherwise be missed. Spatial transcriptomics opens new avenues in cancer research, developmental biology, and neuroscience, providing a detailed molecular map of complex tissues.

Enhancing Spatial Transcriptomics with MMI Single Cell Solutions

Precision Mapping with LMD 

Laser microdissection (LMD) is essential for spatial transcriptomics, as it allows researchers to isolate single cells or tissue regions while preserving spatial context. Unlike conventional methods that dissociate tissues and lose positional information, LMD maintains both the molecular content and the tissue architecture. This ensures that gene expression can be mapped accurately to its exact location, enabling detailed analysis of cellular interactions, heterogeneity, and disease mechanisms at the single-cell level. Using LMD transforms spatial transcriptomics into a precise and reliable tool for modern molecular research.

Isolate cells below 5 µm

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CellCut: Spatial Transcriptomics Made Easy

The MMI CellCut LMD system is perfectly suited for spatial transcriptomics. It preserves the tissue structure, ensuring spatial information remains intact, while enabling highly precise, contamination-free isolation of single cells or very small regions—even subcellular structures. This precision allows researchers to target exactly the areas of interest for downstream molecular analyses, maintaining both RNA and protein integrity.

95%+ Collection Efficiency

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High-Throughput Spatial Transcriptomics with CellCut HT

The CellCut HT isolates single cells or tissue regions into 96-well plates preserving spatial context and RNA integrity. This high-throughput, contamination-free approach makes advanced spatial transcriptomics workflows faster, more reliable, and ideally suited for both small and large studies.

Isolate 96 samples in 10 minutes

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Procedure

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Sample Preparation and Imaging

Mount your sample on a membrane slide, perform all standard staining methods such as H&E, IF, or IHC, and create whole slide images if needed.

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Target Selection

Identify ROIs on the image either by manually marking the cells or automatically using cell segmentation software.

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Cell Isolation

Isolate your ROIs. Dissect hundreds or just a single cell into reaction tubes or 96-well plates. The isolation process is contamination-free and gentle, ensuring that the tissue integrity is preserved.

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Spatial Omics Analysis

The isolated cells undergo a variety of RNA sequencing approaches. These analyses provide insights into the transcriptome and epitranscriptome, highlighting gene expression and RNA modifications.

Revolutionising Pathology:

Why Mark Kühnel Trusts the MMI CellCut

Your Challenge,

Our Expertise

We work hand-in-hand with you, designing solutions that fit your unique research needs. Complex samples? Customized workflows? We handle it all—quickly and efficiently.

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